DEV02440 Supplementary Material

Files in this Data Supplement:

• Supplemental Tables - Adobe PDF

• Supplemental Figure 1 -

  Fig. S1. Methylcellulose colonies derived from E7.5 embryos. (A) Photographs of methylcellulose colonies and cells composing colonies are representatively shown.  (b) Cells composing a colony (a) are shown. The arrow indicates a macrophage; the white arrowhead indicates immature erythroblasts; the black arrowhead indicates nucleated erythrocytes. (d) Cells composing a colony (c) are shown. The arrow indicates a megakaryocyte. (B) RT-PCR analysis for βH1 and β-major globin gene expression in individual hematopoietic colonies.

• Supplemental Figure 2 -

  Fig. S2. Oct3/4-GFP expression in an E7.75 embryo. An Oct3/4-GFP^–/– embryo (left) and an Oct3/4-GFP^+/– embryo (right) at the late allantoic bud stage in bright light (left panel) or fluorescent light (right panel) are shown. Scale bar: 100 μm.

• Supplemental Figure 3 -

  Fig. S3. Genes expressed in Oct3/4-GFP⁺ or Oct3/4-GFP^– cells. PCR was performed on normalized cDNA derived from Oct3/4-GFP⁺ cells and Oct3/4-GFP^– cells in E7.5 embryos.  Positive control cDNA was prepared from day 3 embryoid bodies.