spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Frequency of endothelial or hematopoietic progenitors in E7.5 embryos. (A) Stroma-dependent hematopoietic colonies (a,b) and vascular endothelial colonies (c,d) are representatively shown. These colonies were formed by cells obtained from E7.5 embryos in co-culture with OP-9 stromal cells. Endothelial colonies were immunostained with anti-CD31 antibody. Red, CD31; blue, DAPI. Scale bar: 100 µm. (B) Limiting-dilution analysis of endothelial progenitors and stroma-dependent hematopoietic progenitors in E7.5 embryos. Cells were plated in 96-well plates and were co-cultured with OP-9 stromal cells for 7 days in the presence of IL3, TPO, SCF, EPO and VEGF. The frequency of endothelial progenitors was estimated to be one in 0.05 embryo equivalents. The 95% confidence interval (CI) was 1/38-1/56. The frequency of stroma-dependent hematopoietic progenitors was estimated to be one in 0.14 embryo equivalent, and the 95% CI was 1/115-1/181.





Right arrow Return to article