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Figure 3


Fig. 3. Roof plate cells do not significantly contribute to the cerebellar anlage. (A) Schematic of the genetic strategy to permanently label progeny of roof plate cells. Black triangles indicate loxP sites. (B) Side view of X-Gal-stained E10.5 r.p.Lmx1a-cre;ROSA embryo. LacZ expression (arrow) recapitulates dorsal Lmx1a expression at E10.5. (C) Sagittal section of the E12.5 anlage of immunostained r.p.Lmx1a-Cre;ROSA embryo. Arrowhead points to LacZ+ cells. Arrows point to Math1+ c1 cells (D) Sagittal section of X-Gal-stained E18.5 r.p.Lmx1a-Cre;ROSA embryo. External granule cell layer (EGL) is marked by broken line and arrows. Arrowhead indicates X-Gal+ choroid plexus.





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