spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. Roof plate ablation causes loss of Wnt1 and c1 populations, and reduces cerebellar anlage proliferation at E10.5. (A) Schematic of the genetic strategy to ablate roof plate cells. (B-I) Whole-mount in situ staining of E10.5 embryos with markers and genotypes indicated. (B,C and H,I) Dorsal views with broken line in C demarking the dorsal edge of the open neural tube. (D-G) Side views. Arrows in B-E indicate position of r1 roof plate. Arrowheads `o' and `v' mark normal otic and ventral midbrain Lmx1a expression in panels B and C. Upper and lower insets in panel E show Wnt1 and Fgf8 isthmic expression. Arrows in panels F and G point to the cerebellar rhombic lip. Arrows in panel H show broad expression of cyclin D2 which is eliminated in roof plate-ablated embryos (I). Weak staining in I is ventral, visible because of the open neural tube. Dark lines on dorsal edges are artifact. (J,K) Schematic illustration of E10.5 wild-type and roof plate-ablated embryos demonstrating the consequences of ablation. (L) Hematoxylin and eosin-stained sections at the level of the horizontal line in panels J and K in time series. Arrows point to the most dorsal neuroepithelium of roof plate-ablated embryo (M-P) Immunostained transverse sections at E10.5 demonstrating that cell proliferation and cyclin D2 expression is reduced across the entire cerebellar anlage in ablated embryos. Arrowheads point to the developing cerebellar anlage.





Right arrow Return to article