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Figure 8


Fig. 8. Effect of roof plate ablation on specification of cerebellar cells at E12.5. (A-D) Whole-mount in situ staining showing c3 and pc2 populations in wild-type and ablated embryos at E12.5. Arrow in A indicates roof plate expression of Lmx1a which is eliminated in panel B. Arrowheads `o' and `v' mark normal otic and ventral midbrain Lmx1a expression in panels A and B. Arrows in C and D demonstrate pc2-specific expression of Ptf1a and the reorientation of the open edges of the dorsal neural tube, illustrated in panels E and F. (G,H,J,K,M,N) Transverse immunostained sections at the level of the horizontal line in panels E and F, with markers and genotypes. c1 and RLS markers are missing in ablated embryos, while pc2, c2 and c3 populations are still present, in reduced numbers and altered positions in the anlage. (I,L) Quantification of pc2, c2 and c3 cells found on transverse sections from wild-type and roof plate-ablated embryos. Bars indicate s.e.m. (O,P) Schematic illustrations summarize gene expression changes.





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