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Fig. 1. Development and cellular anatomy of the mouse organ of Corti.
(A-E) Cochlear epithelia from E12.5-E17.5 from mice carrying a
transgenic Math1-GFP reporter (Chen et
al., 2002; Lumpkin et al.,
2003). Epithelia were dissected and photographed under brightfield
conditions and fluorescence images of Math1/GFP expression (green) from the
same preparations were superimposed to illustrate the basal to apical pattern
of hair cell differentiation that initiates in the mid-basal regions of the
cochlea at E14.5 (Chen et al.,
2002) and spreads apically until 
E17.5 when the wave of
differentiation is complete. Note that at E12.5 and E13.5 Math1/GFP expression
is limited to the saccule where Math1-dependent hair cell differentiation
begins around E10.5 (Bermingham et al.,
1999). (F) Cross-section through a P1 organ of Corti
illustrating the position of hair cells (green, stained with antibody to
Myosin VIIa) and supporting cells (red, stained with antibody to
p27Kip1). The different varieties of hair cells and supporting
cells are labeled: I, inner hair cell; O1-O3, outer hair cell-row 1-3; IP,
inner phalangeal.
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