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Figure 4


Fig. 4. UAS osk-K10 produces Oskar protein before oogenesis arrest in oskA87/Df(3R)pXT103. (A) Scheme showing transgene structure and priming sites of PCR primers used for Real-time PCR. Large blue boxes represent oskar coding region. Grey and orange thin boxes represent oskar and K10 UTRs, respectively. Thin lines correspond to introns. Oskar specific primers are in red, K10 specific primer is in green. (B) Genotypes of ovaries analyzed. Numbers correspond to numbers in panels A, C. (C) Two percent agarose gel showing end products of Real-time PCR reactions performed in triplicate using cDNA of early stage ovaries of genotype numbered on top. bicoid (bcd) was used for normalization. UAS oskK10 is about 15 fold less abundant than UAS osk WT. NTC: non-template control. (D) Antibody staining of non-rescued ovaries from females expressing UAS oskK10 (genotype #1) show that Oskar protein (green) is expressed as early as in germaria. F-actin and DNA are in red and blue, respectively.





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