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Figure 5


Fig. 5. The oskar 3'UTR is sufficient for Staufen transport to the oocyte, but not for oskar RNA localization at the posterior pole. (A-C) Staufen revealed by immunofluorescence in stage 4 oskA87/Df(3R)pXT103 egg chambers expressing pCogGal4:VP16;NosGal4:VP16-driven UAS oskWT (A), UAS oskK10 (B), and UAS osk3'UTR (C). (D) The karyosome defect is rescued in egg chambers expressing pCogGal4:VP16;NosGal4:VP16-driven UAS osk3'UTR. White arrow marks an intact karyosome revealed by DAPI stain. (E-H) Top and bottom panels show stage 10 oskA87/Df(3R)pXT103 egg chambers expressing UAS-driven oskWT and osk3'UTR, respectively. (E,F) Staufen detected by immunofluorescence is shown in green; DAPI staining is in red. (G,H): oskar RNA detected by fluorescent in situ hybridization is shown in red; DAPI staining is in blue.





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