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Fig. 6. MAK inhibits Wnt signaling. (A,B) Axis-inducing
activity of Dsh is suppressed by MAK, but not MAK-KD RNA. (A) Indicated RNAs
(2 ng) were injected into a single ventral cell of four-cell embryos and
secondary axes were assessed at stages 35-38. (B) Summary of axis induction
assays, demonstrating that MAK, but not MAK-KD, inhibits Dsh signaling.
Partial axes contained secondary neural tubes, usually up to the hindbrain
level, without eyes, complete secondary axes contained eyes and/or cement
glands. (C) MAK inhibits pSiaLuc reporter activation induced
by Xwnt8 and Dsh, but not by ß-catenin. Luciferase activity was
determined in lysates of embryos injected with 20 pg of pSiaLuc and
indicated mRNAs. Results shown in relative light units represent means of
three samples±s.d., with five embryos in each sample.
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