Supplemental Figure 1
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Fig. S1. Mitotic activity of B7.3 and B8.6 blastomeres persists in SB431542- and DAPT-treated embryos, respectively. (A,B) B7.3 blastomeres of control (A) and SB431542-treated (B) embryos were labelled with DiI at the 64-cell stage and their descendents were observed at the early tailbud stage. (A′,B′) Confocal images of the embryos shown in A,B, respectively. (C,D) B8.6 blastomeres of control (C) and DAPT-treated (D) embryos were labelled with DiI at the 76- to 110-cell stages and their descendents were observed at the early tailbud stage. (C′,D′) Confocal images of the embryos shown in C,D, respectively. DAPI in blue and DiI in red. In these experiments we confirmed the loss of secondary notochord by analysing Ci-Bra expression in B8.6 at the early gastrula stage (0% expression, n=30 for SB431542; 16% barely detectable expression, n=38 for DAPT).
