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Fig. 5. Ci-Delta2 expression at the 64-cell stage is a target of Nodal
signalling. (A-C) Embryo treatment is indicated above the panels.
n=total number of embryos analysed. (A) Expression of
Ci-Delta2 at the 64-cell stage. The expression pattern is represented
by schematic drawings on the right of each embryo panel. Ci-Delta2 is
expressed in A7.8 (orange), A7.6 (mauve) and b7.10/b7.9 (green). On the
schematic drawings, the secondary notochord precursor is marked by a blue dot.
The graph shows the percentage of embryos (y-axis) showing expression
in at least one blastomere for each of the different lineages (indicated by
the colour scheme), following the treatment shown on the x-axis.
(B) A graph showing the percentage of embryos (y-axis) in
which Ci-Delta2 expression was detected in the different lineages on
the left-(L) or right-(R) hand sides on the embryo following the treatments
indicated on the x-axis. The colour scheme is the same as in A.
Consistent with the observation that Ci-Bra and Ci-Nodal was
sometimes recovered following b5.3 ablation, we observed expression of
Ci-Delta2 in the trunk lateral cell precursor on the ablated side in
11% of embryos when analysed at the 76-cell stage (n=53). (C)
A representative embryo for each of the treatments shown in B.
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