|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Ci-Delta2 encodes a Delta-like molecule. (A)
Alignment of the putative DSL domain of Ci-Delta2 with DSLs from other Delta
proteins. Ci, Ciona intestinalis; lv, Lytechinus variegatus
(green sea urchin); Z, zebrafish (Danio rerio); C, chick; Dm,
Drosophila melanogaster; spider, Cupiennius salei. The DSL
domain of Ci-Delta2 was identified using the SMART programme, but gave a score
less significant than the required threshold to be confidently predicted as a
DSL domain using this programme (Letunic
et al., 2004; Schultz et al.,
1998). (B) Ci-Hes-b expression at the early
gastrula stage is a target of Ci-Delta2. Expression of Ci-Hes-b is
shown following the treatment indicated above the panels. Ectopic expression
was sometimes observed in the mesenchyme lineages following injection of
dnDel2 (far right). The graph shows the percentage of embryos
(y-axis) showing expression in at least one cell of the A-line
(A8.16, A8.8, in grey) and B-line (B8.6, in blue) following the treatments
indicated on the x-axis. n=total number of embryos examined.
The schematic drawing shows the positions of these blastomeres. Dots indicate
the blastomere derivatives that were expressing Ci-Delta2 at the
64-cell stage. At the early gastrula stage, Ci-Delta expression is
downregulated in A7.6, while that in A8.15/A8.16 (daughters of A7.8) becomes
stronger (Hudson and Yasuo,
2005).
|
