DEV02478 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 - Fig. S1. Gdf11 expression in chick embryos. Gdf11 expression patterns in HH stage 14 (A), stage 15 (B), stages 17 (C) and stage 21 (D) chick embryos. (i-iv) Cross-sections at corresponding levels indicated in B,D. Green arrows indicate the rostral boundary of the caudal Gdf11 expression domain and red arrows indicate the caudal boundary of the rostral Gdf11 expression domain in the spinal cord. Scale bars: 0.5 mm in A-D; 100 μm in i-iv.

• Supplemental Figure 2 -

  Fig. S2. Neuronal differentiation in Gdf11 and Fst electroporated embryos. (A,D) LH2, (B,E) Lim1/2 and (C,F) Isl1/2 expression in cross-sectioned spinal cords from HH stage 25 embryos 3 days after Gdf11 (A-C) or Fst (D-F) electroporation. The electroporated side (on the right in all panels) is marked by GFP expression. (A,B) The same section; and (D,E) the same section. Scale bars: 100 μm.

• Supplemental Figure 3 -

  Fig. S3. Gdf11 expression in tissues used for western analyses. (A) Dorsal view of a segment from an HH stage 15 embryos 1 day after Gdf11 electroporation. The electroporated (bottom) and the control (top) sides were separated and used for western analysis. (B) Gdf11 expression in an E9.5 Gdf11^+/+ mouse embryo. Gdf11^+/+, ^+/− and ^−/− littermates at this stage were collected for western analysis. Scale bars: 0.5 mm