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Figure 5


Fig. 5. Caudal displacement of Hox expression domains, motoneuron column and pool positions in Gdf11-/- spinal cord. (A-D) Hoxc8 and Hoxc10 expression in flat-mounted open-book preparations of spinal cords isolated from E12.5 control (A,C) and Gdf11-/- (B,D) mouse embryos. Rostral is towards the top and caudal is towards the bottom. The caudal boundary of Hoxc8 expression is indicated by arrows in A,B, while the Hoxc10 expression domain is located between arrows in C,D. (E-H) Raldh2 and Pea3 expression in flat-mounted open-book preparations of spinal cords isolated from E13.5 control (E,G) and Gdf11-/- (F,H) mouse embryos. Rostral is towards the top and caudal is towards the bottom. Arrows indicate the expression boundaries of Raldh2 and Pea3. Scale bars: 1 mm.





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