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Figure 2


Fig. 2. Rescue of sma-9 mutant phenotypes by sma-9 genomic-cDNA hybrid constructs. (A) Schematic diagrams of the predicted full-length sma-9 genomic region and the three genomic-cDNA sma-9 hybrid constructs generated, with exons 1, 4, 6, 9 and 20 labeled. Diagrams are drawn roughly to scale. Yellow ovals represent zinc fingers. The gray box indicates the unique 70 amino acid domain of C2. Broken red outline highlights regions containing genomic sequences. Broken blue outline highlights regions containing cDNA sequences. The broken green line indicates the region targeted by RNAi using pJKL619 (RNAi-N). The broken purple line indicates the region targeted by RNAi using yk127d10 (RNAi-C). Positions of cc606 and cc604 mutations are shown, as well as the region deleted in the tm572 allele. (B) Rescue by sma-9 genomic-cDNA hybrid constructs. All constructs were injected into sma-9(cc604) animals to test for rescue. Rescue was scored using CC::GFP in at least 50 F1 transgenic animals and verified in multiple transgenic lines. +++, at least 50% rescuing efficiency; ++, 25%-50% rescuing efficiency; -, no rescue.





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