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Figure 4


Fig. 4. Inhibitory effect of Wnt11 on the expression of ectodermal Wnt6. (A) Electroporation of Wnt11 RCAS constructs along with GFP plasmids leads to the upregulation of Wnt11 expression in somites (arrowheads). (B) Fluorescence image of the embryo in A showing the localization of the constructs electroporated. (C) Overexpression of Wnt11 leads to the loss of Wnt6 expression in the ectoderm (arrowheads). (D) Embryo in C viewed under fluorescence light showing the position of the electroporated Wnt11 construct. (E) Transverse section of the operated region of embryo in C, showing the loss of Wnt6 expression in the ectoderm (arrowhead). (F) Overexpression of dnWnt11 results in the upregulation of Wnt6 expression in the ectoderm (arrowhead). (G) Localization of dnWnt11 plasmid shown by fluorescence emitted by the GFP plasmids that were co-electroporated. (H) Transverse section of the embryo in F at the operated site, showing a lateromedial extension of Wnt6 expression (arrowheads).





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