|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 4. LMX1B and WNT1 slowly induce GDF7 expression. Lmx1b and
Wnt1 are competence factors for RP differentiation. At stage HH10-11,
Lmx1b (A) and Wnt1 (B) are expressed both on
the dorsal midline and widely in the caudal midbrain (between arrowheads).
Lateral views (C,E,E') and transverse sections of
the midbrain (D-D'',F) of embryos fixed 10 (D-D''), 24
(C) and 34 (E,E',F) hours after coelectroporation of Lmx1b and
Egfp expression vectors. Lmx1b (C,E,F) or Wnt1
(D-D'') transcripts are detected in red, Gdf7 transcripts in
purple (C,D'',E,F) and EGFP in green (D',E'). Twenty-four
hours after electroporation, very few scattered cells express Gdf7
ectopically (C). Gdf7 expression is strongly induced 34 hours after
electroporation (E,F). Except in the caudal midbrain (arrowhead in E) and at
the midbrain-forebrain junction, the purple staining for GDF7 partly obscures
that for Lmx1b (E,F). This fainter induction of GDF7 does not result
from a difference in the efficiency of Lmx1b electroporation around
the constrictions, as the co-electroporated EGFP reporter is expressed at high
levels in the same regions (arrowhead in E').
|
