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Fig. 2. dizzy is required for proper cell migration and cell shape of embryonic macrophages. (A,C,E,G) Hemizygous dizzyⁿ mutant embryos; (I,K) homozygous dizzy¹ mutant embryos; (B,D,F,H,J,L) embryos, wild-type for dizzy; (M) embryo carrying srph-Gal4 UAS-ds.dizzy. (A,E,I,K) Mutations in dizzy affect the migration of macrophages. (A) At stage 14, the posterior part of the ventral nerve cord (VNC) lacks macrophages in dizzy mutants (between red arrowheads), whereas in wild-type embryos (B), the VNC is completely surrounded by macrophages. (E) In the dizzy mutants the gap eventually disappears toward stage 15. (F) Wild-type embryos have an even distribution of macrophages around the VNC at this stage. (C,D,G,H) In addition, dizzy mutant macrophages form smaller protrusions than wild-type cells, here shown for macrophages migrating along the dorsal edge of the epidermis at stage 14 (C,D) and at lateral position at stage 15 (G,H). At this stage there are fewer macrophages in lateral positions in mutant embryos (G) than in wild-type (H). (I,K) In dizzy mutants, macrophages become trapped beneath the amnioserosa (white arrowhead, dorsal view). The hindgut primordium is devoid of macrophages (arrow, I, dorsal view; K, sagittal view). (J,L) In wild-type embryos, macrophages effectively enter the posterior end and leave the space between amnioserosa and yolk. A fraction of them surrounds the developing hindgut (arrow, J, dorsal view; L, sagittal view). (M) dizzy function is required in macrophages for proper cell migration. In embryos, expressing a fragment of dizzy dsRNA in macrophages, these cells do not populate the posterior nervous system at stage 14 and leave a gap (between red arrowheads), which is not entirely closed later. Like in the mutants, macrophages become trapped between yolk and amnioserosa (white arrowhead). Embryos with srph>cd2 and stained for CD2 to visualize macrophages; anterior to the left and dorsal up. For A-H, each picture consists of serveral merged confocal images representing a slice of about 12 µm thickness.

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