DEV02450 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 - Fig. S1. Local patterning defects in the telencephalon and hypothalamus, in ne2611 mutants compared with their wild-type siblings. (A,C,E,G,I) Wild-type siblings; (B,D,F,H,J) ne2611 mutants. All views are lateral (except for C,D: dorsal views), anterior left, at the stages indicated. In ne2611 mutants, the expression domains of the ventral and proximal telencephalic markers dlx2a (A,B) and tbr1 (C,D, insets are lateral views of the same embryos) are enlarged but normally organized, the telencephalic but not epiphyseal (ep) expression of flh is absent (E,F, arrowhead) and that of emx2 is disorganized (G,H, arrows). The anterior hypothalamus, revealed by nkx2.1b (arrows in I) is reduced (arrowheads in J).

• Supplemental Figure 2 -

  Fig. S2. Expression of the early telencephalic marker hesx1. (A-D) Expression of the early telencephalic marker hesx1 in wild-type embryos (A,C) compared with embryos injected with rx3 (B) or rx3^ne2611 (D) mRNA at the stages indicated. All views are dorsal, anterior left. Note that rx3, but not rx3^ne2611, reduces hesx1 expression. An identical result is obtained when probing for tlc expression (not shown).