DEV02465 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Serial coronal sections of the E12.5 Fgfr1^–/–;Fgfr2^–/– mutant telencephalon analyzed by RNA in situ hybridization using probes for Foxg1, Dlx2 and Foxd1. There is no overlap between the telencephalic Foxg1 expression domain and the remaining Dlx2 expression domain, and Dlx2 expression coincides with expression of the hypothalamic marker Foxd1 (arrowheads indicate the borders of expression).

• Supplemental Figure 2 -

  Fig. S2. The E18.5 Fgfr1^–/–;Fgfr3^–/– mutant lacks NPY-positive cells. Boxed areas, along the normal migratory pathway of NPY-positive cells, are enlarged in A-F. NPY-positive cells are readily detectable in all areas of the control (arrowheads, A-C), but are missing in the mutant (D-F). Scale bar: 0.1 mm.

• Supplemental Figure 3 -

  Fig. S3. The Fgfr1^–/–;Fgfr2^–/–;Gli3^–/– telencephalon is dorsalized. RNA in situ hybridization of serial coronal sections. Emx2 expression is found in Foxg1 expressing areas, whereas Dlx2 is not. Scale bar: 0.5 mm.