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Fig. 3. Migration of mesoderm into TC/TC
allantoises from the primitive streak is normal, but proliferation, cell
number and allantoic length are reduced. (A) Whole-mount images of
the allantois (al) and primitive streak (asterisk) at the time of (a,d) and 12
hours after (b,c,e,f) injection of DiI (red) into the primitive streak of +/+
(a-c) and TC/TC (d-f) EHF conceptuses.
(a,b,d,e) Live images of the posterior end (up) through the yolk sac; (c,f)
live images of the dorsal allantoic surfaces after the yolk sac was peeled
away. The green line in a,b,d,e represents the boundary between the allantois
and primitive streak; the yellow line in c,f outlines the extent of the
allantois. Scale bar in d: 500 µm for a-e, 250 µm for f. al, allantois;
ch, chorion. (B) Mitotic index (MI) calculated for the allantois (a)
and primitive streak (b) for +/+, TC/+ and
TC/TC genotypes over the 
6-hour window
during which allantoic length and cell number were reduced in
TC/TC. Error bars represent s.e.m.;
P-values for each mutant genotype with respect to +/+ are reported
below each data point. (C) Bar graph of allantoic nuclei counts in +/+,
TC/+ and TC/TC genotypes
for the interval LB to 1-s. (D) Bar graph of allantoic lengths (in
µm) in +/+, TC/+ and
TC/TC genotypes for the interval EHF to 1-s. In
C and D, error bars represent the s.e.m.; P-values for the mutant
genotypes with respect to +/+ are reported above each bar.
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