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Fig. 2. Whole-mount in situ analysis of XHRT1, esr9,
esr10, Xhairy1 and Xhairy2b in comparison with
X-Delta-1 and X-Serrate-2 in the pronephros region
(arrowheads) of late tailbud to tadpole stage embryos. Nieuwkoop-Faber
stages are indicated. (A) At stage 25, XHRT1 is expressed in
the most dorsoanterior portion of the pronephros anlagen. (B)
Transversal section of the embryo shown in A at the level indicated.
(C-P) Expression of XHRT1, esr9, esr10, Xhairy1, Xhairy2b,
X-Delta-1 and X-Serrate-2 in stage 28 embryos. (A,C,E,G,I,K,M,O)
Whole embryos, lateral views; (B,D,F,H,J,L,N,P) transversal sections of the
corresponding embryos at the level indicated. Note that esr9 and
esr10 staining appears slightly ventral compared with XHRT1.
While X-Delta-1 expression is restricted to a band just below
XHRT1, Serrate2 expression is broader. (Q,R) Double
labeling of XHRT1 and X-Delta-1 or Xhairy2b.
(S,T) At stage 35, Xhairy2b is co-expressed with
X-Serrate-1 in the proximal and distal tubules. dt, distal tubules;
pn, pronephros; pt, proximal tubules; m, medial intermediate mesodermal
layer.
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