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Fig. 1. Regulatory landscape of the Abd-B gene of the BX-C.
(A) Genomic map of the 3' cis-regulatory region of the
Abd-B gene. The proximal and distal arrows point towards the
centromere and telomere, respectively. The black numbers above the DNA line
correspond to the nucleotide sequence marked in kb (SEQ89E)
(Martin et al., 1995). The
exons of the Abd-B transcription unit encoding the m variant are
shown below the DNA line. The extent of the iab-5, 6 and 7
cis-regulatory domains are indicated by brackets, with the domain boundaries
in between shown by gray circles. The red boxes on the DNA line mark the IAB5,
IAB6, IAB7a, IAB7b and IAB8 initiator elements (from proximal to distal,
respectively). The extent of the deletions analyzed are shown below the DNA
line, in black for deletions associated with loss-of-function of phenotypes
and in red for gain-of-function phenotypes. (B) The DNA line with the
scales as in A is drawn again. The extent of the different restriction
fragments used in transgenic constructs are shown below, with the initiator
fragments drawn in red. The expression patterns directed by these initiator
fragments are presented in C-F, which show embryos at the extended germ
band stage, doubled stained with antibodies directed against Engrailed (brown)
and the ß-Galactosidase (in blue). The parasegmental anterior border of
lacZ expression (indicated by an arrow) shifts one parasegment
posterior in each panel from left to right
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