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Fig. 3. The metanephric mesenchyme fails to form in Odd1-/-
embryos. (A,B) Sections through the metanephric region of
E11.5 heterozygous and homozygous null embryos stained with hematoxylin and
eosin. In heterozygotes (A), a nephric duct, ureteric bud and condensed
metanephric mesenchyme are seen. Homozygous null embryos (B) completely lack a
ureteric bud and condensed mesenchyme (arrow). A duct is often present (on
right side but not left in B), but smaller than normal. The region between the
aorta and nephric duct is also significantly smaller in the Odd1 null
embryos. (C-J) Sections through the metanephric region of mouse embryos
stained by in situ whole-mount hybridization (C-F) or immunofluorescence (G-J)
for the indicated gene products (Dapi nuclear stains in G and I correspond to
sections in H and J, respectively). At E10.5, the metanephric mesenchyme of
Odd1 heterozygous embryos expresses Six2 (C), Eya1
(E) and Pax2 (H). All three gene products are absent from the metanephric
mesenchyme region of Odd1 null mutants (D,F,J). The mutant nephric
duct does express Pax2 (J). The duct in mutant embryos is outlined with a
dotted line (D,F,I). The fluorescence in the upper right corner in J is
autofluoresc;ence from blood cells in the aorta. a, aorta; d, nephric duct;
mm, metanephric mesenchyme; u, ureteric bud.
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