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Fig. 5. Genetic interactions of Sall4 and Sall1.
(A) Bilateral renal agenesis in Sall1/4 heterozygotes. Six out
of the 38 compound heterozygotes analyzed had this phenotype, while 10 had
unilateral agenesis. a, adrenal glands; b, urinary bladder; c, colon; k,
kidney. (B) Anal stenosis in Sall1/4 heterozygotes at E17.5
(right two panels). Black arrowhead shows complete stenosis of the rectoanal
junction; white arrowhead shows absence of the rectum. a, anus; b, urinary
bladder; r, rectum. (C) In situ hybridization of Sall4 and
Sall1 at E8.5. The upper side is the anterior region of the embryo
(transverse section). Black and white arrowheads indicate the mesenchyme and
neuroepithelium, respectively. (D) Sall4 and Sall1
expression in the anorectal region at E11.5 (arrowheads). Heterozygotes of
Sall4-ßgeo and Sall1-lacZ
(Nishinakamura et al., 2001)
were stained using X-gal. (E) Overlap of Sall4 and
Sall1 in the developing heart at E11.5. Sall4 is expressed
in myocardium (arrowhead), while Sall1 is expressed in myocardium
(arrowhead) and endocardium (arrow). Sall4-ßgeo and
Sall1-lacZ mice were stained using X-gal. (F)
Immunocytochemistry of Sall4 and Sall1, and counterstaining with DAPI in ES
cells. (G) Binding of Sall4 and Sall1 shown by immunoprecipitation
using ES cell lysates.
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