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Fig. 2. Activity of the Pitx2 ASE from various vertebrates. Reporter constructs (hsp68-lacZ) driven by the Pitx2 ASE from various vertebrates were injected into fertilized mouse eggs, and the resulting embryos were stained with X-gal. (A-E) Frontal and lateral views of transgenic mouse embryos at E8.2. All constructs examined gave rise to asymmetric X-gal staining in left LPM, although the zebrafish ASE was active only in the anterior portion of left LPM. (F-Y) Transverse sections of embryos at E10.5. Activities in the common atrial chamber (F-J), foregut (lung bud) (K-O), septum transversum (future liver) (P-T), and gut dorsal mesentery (U-Y) are shown. Red arrows indicate the domains in which the ASE was specifically active. Encircled + or - signs indicate whether the ASE was active or inactive, respectively. The ASE from mouse, human or chicken Pitx2 showed left-sided activity in the indicated organs. The frog ASE was active in the common atrial chamber and gut dorsal mesentery, but inactive in the foregut and septum transversum. The zebrafish ASE was inactive in all organs. The number of the embryos examined and variations in expression patterns are shown in Table S1 of the supplementary material. Scale bars: 200 µm. A, anterior; L, left; P, posterior; R, right.

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