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Fig. 4. Generation of mice that lack the ASE of Pitx2. (A)
Schematic representation of the mouse wild-type Pitx2 allele, a
targeting vector designed to delete the 0.6 kb region of Pitx2 that
contains the ASE (red oval), as well as the targeted allele before and after
Cre-mediated recombination. Pitx2 contains six exons (black boxes)
and two promoters, with transcription beginning at exon 1 or exon 1c.
(B) Southern blot analysis of Nsi-digested DNA from ES cells of the
indicated Pitx2 genotypes before Cre-mediated recombination. The
probe used for hybridization is shown by the blue bar on the top. The
wild-type and mutant alleles give rise to hybridizing fragments of >23 and
11 kb, respectively. Green triangles, loxP sequences; neo, neomycin
resistance gene under the control of the phosphoglycerate kinase gene
promoter; DT, diphtheria toxin gene.
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