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Figure 8


Fig. 8. Regulation of Pitx2 expression by negative feedback in the ventricle and AV canal of the heart. (A-H) Expression of the Pitx2 17-lacZ transgene in the heart of wild-type (A-D) or Pitx2{Delta}ASE/{Delta}ASE (E-H) embryos at E9.5. (I-P) Expression of the Pitx2-Cre transgene in wild-type (I-L) or Pitx2{Delta}ASE/{Delta}ASE (M-P) embryos at E9.5 as monitored with the CAG-CAT-lacZ transgene. A left view of the heart is shown in the left-most panels. Red dotted circles indicate a large portion of the ventricle and AV canal in which X-gal staining is absent in A but present in E, I and M. The transverse sections of the heart at the planes indicated by the yellow and red arrowheads are shown in B,F,J,N and C,G,K,O, respectively. The right-most panels show sagittal sections at the AV canal. Their planes are indicated by the red vertical lines in A,E,I,M. The boundaries between SAVC and IAVC in D,H,L,P are indicated by the orange dotted lines. It should be noted that X-gal staining is apparent only at the myocardium adjacent to the SAVC in D, while it is also apparent in the myocardium adjacent to the IAVC (H,L,P). The domain that is subject to the negative feedback regulation is indicated by the yellow bar in D,H,L,P. Scale bars: 200 µm. la, left atrium; lv, ventricle of the heart.





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