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Fig. 9. Generation of transgenic mice that express Pitx2c under the
control of the ASE. (A) Construction of two transgenes. Expression
of the Pitx2c cDNA and lacZ is driven either by the
wild-type ASE or a mutant version (NmASE) that lacks the Nkx2-binding site.
Permanent transgenic mouse lines were established for the former (Tg39,
Tg55) and latter (Tg50) transgenes. Red and blue ovals represent
Foxh1-binding sites and the Nkx2-binding site, respectively. (B-M)
Expression of the transgenes in each transgenic line was monitored by X-gal
staining. The transgenes in each line (Tg39, Tg55, Tg50) were
expressed at similar levels in left LPM at E8.2 (B-D). The transgene of line
Tg39 was asymmetrically expressed in the truncus arteriosus at a high
level (E), in gut dorsal mesentery at a low level (K) and not at all in the
lung bud (H) at E10.5. Asymmetric expression of the transgene in line
Tg55 was apparent at a high level in gut dorsal mesentery (L), at a
low level in the truncus arteriosus (F) and not at all in the lung bud (I).
Asymmetric expression of the transgene in line Tg50 was not detected
in any organ examined (G,J,M). Frontal and lateral views of embryos are shown
in B-D, and transverse sections are shown in E-M. Scale bars: 200 µm.
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