spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. MTS24 co-staining with described markers for epidermal stem cells. (A-C) Frozen section of dorsal skin showing expression of keratin 14-Cy3 (A; red) and MTS24-FITC (B; green) and merged (C) in hair follicle. Arrowhead highlights co-localisation of keratin 14 expression and MTS24 labelling. (D-F) Expression of CD34-Cy3 (D; red) and MTS24-FITC (E; green) and merged (F) in dorsal skin. Note that CD34 expression (arrowhead) was found in a different location within the hair follicle than MTS24-staining (asterisk). (G-I) Wholemount of tail epidermis showing no co-localisation between keratin 15-FITC (G; green) and MTS24-Cy3 (H; red) labelling within the hair follicle. Merged image is shown in (I). (J) Labelling with MTS24-Cy3 (red) and FITC anti-mouse (green) secondary antibody alone, shows that staining of sebaceous gland is non-specific in (G,I). (K) Wholemount of tail epidermis showing {alpha}6 integrin-FITC (green) and MTS24-Cy3 (red) co-staining (arrowhead). SKH-1 (L,M) or wild-type (N,O) neonatal mice received repeated injections with BrdU to generate label-retaining cells. Frozen sections (L-N) or tail wholemounts (O) were collected at 1 day (L), 6 weeks (M) or 10 weeks (N,O) after the last injection with BrdU. Tissue was labelled for BrdU-FITC (green) and MTS24-Cy3 (red). Nuclear counterstain was DAPI (L,M,N; blue). Arrowheads indicate BrdU label-retaining cells (L-O). In each panel the bulge area is bracketed. SG, sebaceous gland; HF, hair follicle; IFE, interfollicular epidermis. Scale bars: 50 µm.





Right arrow Return to article