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Fig. 4. 
6 integrin+/MTS24+ keratinocytes form large colonies with high
efficiency in culture. Keratinocytes harvested from dorsal epidermis of
adult C57Bl/6 mice were analysed by FACS (A,B) or were sorted (C-F) under
sterile conditions. (A,B) Flow cytometric analysis of (A)
isotype control versus (B) MTS24-labelled keratinocytes indicates that there
is a subpopulation of MTS24+ keratinocytes. (C-F) Sorted 6
single+, 6+/MTS24+ or the unseparated mixture (all sorted)
keratinocytes were grown for 14 days, fixed in 4% formal saline, and stained
with Rhodamine B to visualise colony growth. Data are shown from a
representative experiment, which was repeated several times with similar
results. (C) FACS selection of keratinocytes. (D) Representative culture
dishes with stained colonies. (E,F) Graphs of colony-forming efficiency (E,
black bars) and size of colonies (E, white bars; F). Bars represent the mean
of at least four replicate culture wells±s.e.m. Asterisks indicate
significant differences of 6+/MTS24-relative to 6+/MTS24+
(P<0.05; unpaired two-tailed t test).
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