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Fig. 5. CD34 and MTS24 identify distinct subpopulations of basal keratinocytes
with high in vitro replicative capacity. (A,B) Keratinocytes
harvested from dorsal epidermis of adult C57Bl/6 mice were sorted under
sterile conditions. (A) Flow cytometric analysis shows that CD34+ basal
keratinocytes are members of a different population of cells than the MTS24+
keratinocytes. Sorted 
6 single+, 6+/MTS24+, 6+/CD34+ or
the unseparated mixture (all sorted) keratinocytes colonies were grown for 14
days to visualise the colonies (B) and compare relative colony-forming
efficiency (C). (D) Individual colonies from 6+/MTS24+
and 6+/CD34+ keratinocytes were passaged and replated at clonal density
for an additional 14 days. (E) A graphical comparison of the size of
colonies derived following passage. Bars represent the mean of at least four
replicate culture wells±s.e.m. Data are shown from representative
experiments that were repeated with similar results.
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