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Fig. 6. MTS24+ and CD34+ basal keratinocytes show similar gene expression
profiles. FACS-sorted
6+/MTS24+ and
6+/CD34+ keratinocytes
were analysed by Q-PCR for expression of a selected group of genes.
6+/MTS24+ and
6+/CD34+ show the same pattern for genes that are
supposed to be lower expressed (red bars) in hair follicle stem cells or whose
expression is enriched (green bars) in hair follicle stem cells. Expression is
normalized to the reference gene (ß-actin) and fold changes for
6+/MTS24+ and
6+/CD34+ keratinocytes are in comparison to
6+/MTS24- and
6+/CD34-keratinocytes respectively. Average data
given are from two independent isolations and Q-PCR was performed in
triplicate per sorted population.