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Figure 1


Fig. 1. Construction of Wnt9a alleles and analysis of mutants. (A) Schematic of the two targeting constructs, which were introduced into the Wnt9a locus on mouse chromosome 11. Floxed allele (fl): exon 2 (Ex2) flanked by loxP sites. lacZ allele: insertion of an IRES-lacZ cassette into exon 2. The 5' and 3' external probes are indicated below the genomic locus map. Restriction enzymes: B, BamHI; N, NheI; RI, EcoRI; RV, EcoRV. (B) Southern-blot on EcoRV-digested genomic DNA from mutant ({Delta}/{Delta} or lacZ/lacZ), heterozygous (+/{Delta} or +/lacZ) and wild-type (+/+) E10.5 littermates from intercrosses of +/{Delta} and +/lacZ heterozygous mice, respectively, hybridized with the 3' genomic external probe. (C) RT-PCR using RNA obtained from E10.5 wild-type and Wnt9a{Delta}/{Delta} embryos, showing the presence of a 1.1 kb transcript in wild type and a shorter 900 bp transcript in Wnt9a{Delta}/{Delta} mutants. (D) Wild-type (WT) and Wnt9a{Delta}/{Delta} mutant newborn littermates. Mutants are slightly smaller (indicated by the shorter black line on the right side) and that they do not have milk in the stomach (arrow).





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