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Figure 9


Fig. 9. ß-catenin levels and phosphorylation state are altered in dfer mutants. (A,B) ß-catenin immunoprecipitated from (A) dfer{Delta}1 and (B) dfergof late stage embryo extracts, probed with anti-arm (ß-catenin) and anti phosphotyrosine. ß-catenin is hypophosphorylated in dfer{Delta}1 embryos (21% relative to yw control embryos) and hyperphosphorylated in dfergof embryos (168% relative to yw). (C) Levels of ß-catenin are reduced in the epidermis of dfergof embryos. DE-Cadherin may also be reduced to a lesser degree. (D) Western blots on total embryo extracts confirm that ß-catenin is depleted in dfergof mutants. (E) Loading control (anti-actin).





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