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Figure 6


Fig. 6. Behavior of WAVE complex components in Nap1khlo mutants. (A) Western blot analysis of WAVE1 protein in E8.5 embryos, normalized to {gamma}-tubulin levels. (B-E) Immunofluorescent staining shows that Sra1 (B,C) and Abi1 (D,E) are localized to the surface of wild-type (B,D) but not Nap1khlo (C,E) primary mesodermal cells. Scale bars in B-E: 25 µm. (F-I) Actin structures in wild-type (F,H) and Nap1khlo (G,I) epithelial (F,G) and mesodermal (H,I) cells, visualized by phalloidin staining. (J,K) Localization of the actin-binding protein cortactin (green) and DAPI (blue) in wild-type (J) and Nap1khlo (K) mutant mesodermal cells. Scale bars in F-K: 50 µm.





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