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Fig. 3. Anther development from stage 4 to stage 8 in the wild type
(Ler) and dyt1 mutant. Locules from anther sections:
(A,C,E,G,I,K) wild type; (B,D,F,H,J,L) dyt1 mutants.
(A,B) Stage 4 anthers. (C,D) Late stage 4 or very
early stage 5. Vacuolization in the dyt1 mutant occurred in more
cells and the vacuoles were larger than those in the wild type.
(E,F) Stage 5 anthers, with more and larger vacuoles in the
tapetum and middle layer of the dyt1 anther (F) than the wild type
(E). (G,H) Stage 6 anthers. The vacuolization in cells of the
mutant tapetum and middle layer became more extensive. The mutant meiocytes
had a much thinner callose layer around them (arrowheads). (I,J)
Stage 7 anthers. Wild-type meiocytes undergo cytokinesis to form tetrads. In
dyt1 anther, the tapetum and middle layer cells were swollen and had
excess vacuolization, and meiocytes generally collapsed before cytokinesis
(arrowheads). (K,L) At stage 8, in the wild-type anther locules,
microspores were released from the tetrad; in the dyt1 anther, almost
all meiocytes degenerated. E, epidermis; En, endothecium; ISP, inner secondary
parietal cells; SS, secondary sporogenous cells; ML, middle layer; T, tapetum;
Ms, meiocytes; Tds, tetrads; Msp, microspores; D-Ms, degenerated meiocytes.
Scale bars: 10 µm.
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