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Fig. 5. The DYT1 expression pattern. (A) Detection of
DYT1 expression using real-time PCR in Ler background.
DYT1 expression was not detected in any vegetative tissues or
stage-12 flower, was detected at low levels in the young inflorescence and
siliques, and was at the highest level in the anther. (B) Detection of
DYT1 expression using real-time PCR in Ler, ems1 and
spl inflorescences. The DYT1 expression was not detected in
spl, but was detected in ems1 at about 17% of the normal
level. (C-K) RNA in situ hybridization with a DYT1 probe.
(C-F,H) DYT1 expression in the Ler background. (C) The
DYT1 signal was detected in the floral meristem. (D) An anther at
stage 4 to early stage 5. The DYT1 signal can be detected mainly
within the newly formed tapetum and meiocytes. (E,H) At late stage 5, a strong
signal is detected in the tapetal cell layer, whereas the signal in the
meiocytes is much weaker. (F) At late stage 6, the DYT1 signal is
greatly reduced, with residual expression in some meiocytes. (I) A
dyt1 mutant anther at late stage 5; the DYT1 signal is low
and non-specific in the entire anther. (J) A spl mutant anther at
late stage 5. The DYT1 signal is at the background level. (K) An
ems1 mutant anther locule at late stage 5. Uniformly weak
DYT1 signal can be detected in meiocytes and little signal in cells
surrounding the meiocytes. (G) The sense control with a Ler late
stage 5 anther. Only background signal is seen. Rt, root; Sm, stem; Lf, leaf;
Se, silique; S12, stage 12 flower; Inf, inflorescence; Ar, anther; WT-Inf,
wild-type inflorescence; ems1-Inf, ems1 inflorescence;
spl-Inf, spl inflorescence; T, tapetum; Ms, meiocytes; I,
indeterminate cells; E-Ms, excess meiocytes. Scale bars: 20 µm in C-K.
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