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Fig. 3. Expression of tbx-35 is specific for the early MS lineage.
(A,B) A reporter tbx-35::GFP transcriptional fusion
shows GFP fluorescence in the two daughters of MS (MSa and MSp) and their
descendants for several divisions. (C) Depletion of mex-1 by
RNAi results in ectopic expression (arrows) of tbx-35 in AB
descendants, consistent with a transformation of the AB granddaughters to MS
(Mello et al., 1992).
(D) Expression of tbx-35::GFP (arrows) in early C descendants,
consistent with a C to MS transformation in pie-1(RNAi) embryos
(Mello et al., 1992).
(E) Depletion of the divergent ß-catenin wrm-1 by RNAi
results in an E to MS transformation
(Rocheleau et al., 1997), and
concomitant expression of tbx-35::GFP in both the E and MS lineages.
(F) Although MS adopts an E-like fate in pop-1(-) embryos
(Lin et al., 1995), expression
of tbx-35::GFP occurred in the early MS lineage of all mutant embryos
examined (n>30). (G) tbx-35 mRNA accumulates in MS
as detected by in situ hybridization. Seventy-eight percent of embryos at this
stage (n=50) showed MS expression and 22% of embryos did not stain.
(H) Ectopic tbx-35 mRNA in a mex-1(RNAi) embryo.
Seventy percent of embryos at this stage (n=54) showed ectopic
expression, 9% showed normal MS-specific expression and 20% did not stain.
(I) Ectopic activation of tbx-35 in E in a
lit-1(t1512) embryo grown at 25°C
(Kaletta et al., 1997).
Sixty-five percent of embryos (n=55) showed expression in MS and E,
22% showed MS-specific expression and 13% did not stain. (J) Normal
expression of tbx-35 mRNA in a pop-1(RNAi) embryo.
Seventy-eight percent of embryos (n=65) showed staining in MS, while
22% did not stain. For these and subsequent embryo images, anterior is towards
the left, and dorsal is upwards, and the eggshell (seen by Nomarski optics) is
shown with a broken blue line. Scale bar: 10 µm.
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