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Fig. 4. Deficiency of MS-derived cell types in tbx-35(tm1789)
embryos. (A) Differential interference contrast (DIC) image showing
the grinder (gr), indicative of MS-derived pharynx, in a wild-type threefold
stage embryo. (B) A ceh-22::GFP reporter (pseudocolored
yellow) shows the fully elongated pharynx with MS- and ABa-derived halves
(Okkema and Fire, 1994).
(C) Terminal glp-1(or178) embryo showing ceh-22
expression in only MS-derived pharynx. (D) Terminal
tbx-35(tm1789) embryo arrested at 1.5-fold elongation, similar in
appearance to med-1,2(-) embryos
(Coroian et al., 2005). The
grinder is absent, and internal cavities (arrows) are observed in 35% of such
embryos, similar to the hypodermis-lined voids in skn-1(-) embryos
(Bowerman et al., 1992).
(E) ceh-22 expression in only ABa-derived pharynx in
tbx-35(-). (F) Absence of pharynx in a glp-1(-);
tbx-35(-) embryo. (G) Wild-type embryo at the 1.5-fold stage.
(H) Expression of hlh-1::GFP in the embryo shown in G
indicates muscle precursors (Chen et al.,
1994). In wild type, an average of 46.8±1.4 cells
(n=10) were scored as expressing at this stage. (I) In
terminal pal-1(RNAi) embryos, muscles made by C and D are not made,
resulting in a loss of posterior hlh-1 expression (arrows; compare
arrows in H). Expression was seen in an average of 20±0.6 cells
(n=33). (J) A 1.5-fold stage tbx-35(tm1789) embryo.
(K) hlh-1::GFP expression in the embryo shown in J shows lack
of signal in the anterior (arrowheads; compare H), while posterior expression
persists (arrows) and a large cluster of hlh-1-expressing cells is
seen near the center (*). An average of 34.8±2.4 cells
(n=10) was scored in these embryos. (L) Terminal
tbx-35(-); pal-1(RNAi) embryos express hlh-1::GFP in only a
small number of cells, with an average of 5.7±0.5 cells
(n=40).
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