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Fig. 7. Overexpression of TBX-35 is sufficient to specify pharynx and muscle
fates. Images depict terminal embryos that received a 30-minute heat shock
at 33°C before the 100-cell stage. (A) Absence of pharynx muscle
(ceh-22::GFP) in a skn-1(RNAi) embryo (0.0±0.0 cells,
n=36). (B) Small numbers of muscle cells (hlh-1::GFP)
made in a skn-1(RNAi); pal-1(RNAi) background (6.7±0.5 cells,
n=47). (C) Pharynx muscle cells are made when tbx-35
is overexpressed in a skn-1(RNAi) background. Thirty-two percent of
embryos (45/141) had an average of 13.8±1.8 cells per embryo, with 10
embryos containing at least 25 cells as shown here, or an overall average of
4.4±0.8 (n=141). (D) An increase in body muscle cells
in a skn-1(RNAi); pal-1(RNAi) background following ectopic activation
of tbx-35 (an average of 13.2±1.7 cells, n=87).
Fifteen percent of embryos (13/87) had at least 30 muscle cells, similar the
embryo shown here. (E) Numbers of pharynx muscle cells
(ceh-22::GFP) in a skn-1(RNAi) background. (F)
Numbers of body muscle cells (hlh-1::GFP) in a skn-1(RNAi);
pal-1(RNAi) background.
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