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Fig. 4. Expression of Dlx2 orthologs in zebrafish, Synodontis and
medaka. (A-C) dlx2b expression is absent at all stages
from the zebrafish oral region, but present in forebrain (f). (D-F)
dlx2a expression is absent from zebrafish oral epithelium at all
stages, but present laterally in jaw mesenchyme (arrows) and forebrain.
(G-L) dlx2a and dlx2b are expressed in tooth germs of
Synodontis, while only dlx2a is expressed laterally in the
jaw mesenchyme (arrows in J,K) and barbels (arrow in L). Both genes are
expressed (arrowheads) in tooth germ epithelium (cytodifferentiation stage
premaxillary germ in G; initiation stage dentary germ in J), and
dlx2b was additionally detected in tooth germ mesenchyme (arrow in
G). Arrowheads indicate one of three premaxillary germs per side in H, one of
two dentary germs per side in K, and one of numerous germs visible in upper
and lower jaws of I,L. (M-O) Medaka dlx2a is expressed in oral
epithelium (arrowheads in M,N) and mesenchyme (arrows in M-O). Transverse
section (M) indicates epithelium expression is in a medial band and sagittal
sections (N,O) reveal that mesenchymal expression is lateral to this.
Indicated stage is before visible signs of tooth initiation. All whole mounts
in ventral view; transverse sections in B,E-G,J,M; sagittal sections in N,O.
Abbreviations as in Fig. 1. f,
forebrain; mx, maxillary process; y, yolk. Scale bars: 100 µm in
A-H,J-K,M-O; 500 µm in I,L.
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