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Fig. 6. Expression of putative Fgf signaling pathway components in
Astyanax and zebrafish. (A-R) fgf8, dlx2b, pax9
and lhx6 expression. (A,D,G,J) Astyanax fgf8 is expressed in
maxillary and mandibular epithelium of the developing mandibular arch
(arrowheads), with expression domains moving medially between 42 and 48 hpf.
(M) Medial limits of fgf8 expression (arrowheads) do not overlap with
dlx2b expression (arrow) when detected simultaneously. (P) Zebrafish
fgf8 expression resembles that in Astyanax. (B,E,H,K)
Astyanax pax9 is expressed predominantly in the maxillary and
mandibular mesenchyme (arrows), but is also found in patches in the oral
epithelium (arrowheads). (N) Simultaneous detection of shh
(arrowhead) and pax9 (arrow) indicates that pax9 mesenchymal
expression is strongest lateral to the developing tooth germ (arrowhead). (Q)
Zebrafish pax9 expression (arrows) resembles that in
Astyanax. (C,F,I,L,O) Astyanax lhx6 is expressed in the
maxillary and mandibular mesenchyme (arrows). (R) Zebrafish lhx6
expression (arrows) resembles that in Astyanax. (J-L) Sagittal
sections of mandible; (N) transverse section. Abbreviations as in
Fig. 1. f, forebrain; h, heart;
mo, mouth opening; y, yolk. Scale bars: 100 µm.
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