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Figure 7


Fig. 7. Reduction of RME-2 and MEX-5 levels in efl-1(n3363) and dpl-1(n3316) mutants. (A-L) Adult germlines from each genotype were dissected, fixed and stained with an antibody to the corresponding protein as labeled. Blue, DNA; red, antibody. Panels G and J are at 10x exposure relative to A. For each antibody and genotype, n>20. The reduced RME-2 and MEX-5 staining seen in efl-1 and dpl-1 mutants occurred in all gonads examined. LIN-3 staining on the oocyte surface disappears upon RNAi of lin-3, whereas the diffuse staining does not (see Fig. S2 in the supplementary material).





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