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Fig. 4. In vivo association of SEU protein with AG 3'
enhancer. (A) Diagram of the AG second intron, which
coincides with an 
3 kb HindIII fragment
(Bao et al., 2004;
Busch et al., 1999;
Deyholos and Sieburth, 2000).
Numbers indicate the nucleotide sequence, with the 5' HindIII
site designated as 1. The location of LFY/WUS-binding sites (back
circles), CArG boxes (diamonds), and BLR-binding sites (triangles) is
indicated. The two `redundant' enhancers defined by KB14 (5' enhancer)
and KB18 (3' enhancer) reporter lines
(Busch et al., 1999), as well
as the position of the AG-5 and AG-3 PCR products, are
shown. The 900 bp AG fragment in the
pAG3'I::LUC reporter is indicated. Drawing is not to
scale. (B) Association of SEU with the AG 3' enhancer
revealed by ChIP with an anti-SEU antibody. AG-5 and AG-3 are PCR products
detecting immunoprecipitated wild-type and seu-3 chromatin,
respectively. The control E1F4P primer amplifies a non-regulatory target of
LUG/SEU. `No Ab' and `
SEU Ab' correspond to chromatin treated without
or with anti-SEU antibodies, respectively.
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