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Fig. 1. Assay for retinal progenitor proliferation in explants and in ovo.
(A) E5.5 retinal explants electroporated with pCAG:CA-ß-catenin or
pCAG:GFP were cultured for 18 hours and exposed to [3H]thymidine
for 6 hours. The percentage of [3H]thymidine+ cells were
quantified following autoradiography. Bars represent the percentage of cells
labeled with [3H]thymidine among the GFP+ population.
Error bars represent the s.d. (B,C) An in vivo assay for retinal
cell proliferation using BrdU (B) and pH3 (C) in an E7.5 retinas infected with
RCAS:CA-ß-catenin or RCAS:GFP. (D-F) Representative images of an
RCAS:GFP-infected retina at E7.5 showing BrdU incorporation (green) and pH3
(red) in the central retina (D), the peripheral retina including the CMZ (E)
and in the ciliary/iris epithelia (F). (G) Relative position of
sections shown in D,E and F and used for scoring in B and C. (H)
Representative image of an E7.5 retinal section showing BrdU labeling (green)
and pH3 (red) in a thin and folded region induced with RCAS:CA-ß-catenin
from the central part of the retina. Le, lens. Scale bar: 75 µm.
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