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Fig. 4. Effects of Wnt signal activation on RPG expression and retinal
differentiation. (A-D) ISH for retinal progenitor markers Chx10
(A,C) and Notch1 (B,D) at E5.5 following injection of RCAS:CA-ß-catenin
(A,B) or RCAS:Wnt2b (C,D). Arrowheads indicate the areas with higher viral
infection. (E-H) IHC of viral gag protein (p27) on the adjacent (A) or
same sections (B-D) to show the areas of viral infection (red). (I-K)
IHC of ß-Tubulin III (green), a marker of ganglion and amacrine cells,
following the introduction of control RCAS (I), RCAS:CA-ß-catenin (J) and
RCAS:Wnt2b (K) harvested at E7.5. Note the partial overlap of
-ß-Tubulin III staining with viral infection (arrow, J), possibly
due to axonal processes produced by uninfected neighboring ganglion cells. IHC
of viral gag protein (p27) shows the area of viral infection (red).
(L,M) IHC for Visinin, a marker for developing photoreceptor
cells, in uninfected control (L) and RCAS:CA-ß-catenin-infected (M)
retinas. (N,O) IHC of -Pax6 (green), a marker for
horizontal (arrow) and amacrine (marked by the upper bar) cells, and cells in
the ganglion cell layer (lower bar), in E7.5 wild-type retina (N) and
RCAS:CA-ß-catenin-infected retina (O). Nuclear counter staining was done
with DAPI (blue). Viral infection is visualized using IHC for viral gag (red).
Scale bars: 75 µm.
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