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Fig. 2. Development of PAX2-positive RF and IB4-positive hyaloid artery
precursors is impaired in Bmp7-/- embryos. (A-P)
Parasagittal sections at different distoproximal levels of E10.5 (A-F)
or E12.5 (G-P) optic cup from wild-type or microphthamic
Bmp7-/- embryos stained with antibodies against PAX2 or
with the isolectin B4 (IB4) as indicated. At E10.5, PAX2-positive staining is
progressively reduced along the distoproximal axis (arrows in D-F), and by
E12.5 is completely absent from the optic cup (arrows, J-L) of Bmp7
null embryos. PAX2-positive RF precursors are absent from the prospective
ventral neural retina in the proximal optic cup from Bmp7-null
embryos (empty arrows in E,K) when compared with the wild type (arrow in B,H).
The ventral invagination of PAX2-positive precursors is nearly absent in the
proximal optic cup of the mutants (empty arrow in F,L) when compared with the
wild type (arrow in C,I). By contrast, PAX2-positive precursors are normally
aligned on the wall of the optic stalk in both wild-type and mutant embryos
(insets in I and L), although the stalk is smaller in the latter. Staining
with the IB4 highlights the presence of hyaloid artery precursors in wild-type
(arrow in N) but not in mutant (arrow in P) embryos, while IB4-positive
vessels are visible in the distal optic cup in both wild type (M) and mutants
(arrow in O). (Q) Schematic drawing summarising the morphological
alterations observed in the mutant optic cup. In the mutant, ventral RF
precursors are diminished and do not invaginate into the ventral optic cup.
pnr, prospective neural retina; prpe, prospective retina pigmented epithelium;
rfp, RF precursors. Scale bar: 50 µm in A,B,D,E; 25 µm in C,F;100 µm
in G-P.
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