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Fig. 6. Cell proliferation and apoptotic cell death are reduced in
Bmp7-/- optic cups. (A-C) Pregnant mice were
injected with BrdU and sacrificed 40 minutes later. Embryos were fixed and
frontal cryostat sections were immunostained with antibodies against BrdU. The
number of BrdU-positive cells present in the dorsal and ventral halves of the
optic cup from wild-type (A) or Bmp7-/- embryos (B) was
calculated. The dorsal border (broken lines) of Vax2 expression
(insets) was considered as the dividing landmark. (C) The graph represents the
percentage of BrdU-positive cells in the ventral and dorsal wild-type (blue)
or mutant (purple) optic cups. The decrease of BrdU-positive cells in the
ventral optic cup of the mutants is statistically significant
(***P<0.0001). (D-I) Parasagittal cryostat
sections at different proximodistal levels of wild-type or mutant optic cups
were processed for TUNEL staining. In wild type, apoptotic cells are
concentrated along the prospective RF (arrowheads in E,F). Apoptotic cells are
almost absent in the equivalent sections from the mutants. Abbreviation: lv,
lens vesicle; ns; no significance. Scale bar: 60 µm (30 µm in
insets).
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