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Fig. 7. BMP7 requirement for RF formation is independent of Chx10
function. Frontal (A-D,G-J) and parasagittal (E,F) vibratome
(A-D,I,J) or cryostat (E-H) sections through the optic cup of
wild-type, Bmp7-/- and orJ embryos (as
indicated) were hybridised with probes specific for Chx10, Bmp7 and
Math5 (as indicated) or immunostained with antibodies against PAX2
and TuJ1 (as indicated). Chx10 expression is unchanged in the
Bmp7-null mice (A,B) and Bmp7 expression is normal in the
orJ mutant (C,D). PAX2-postive RF precursors are initially
present in the orJ mutants (arrow in F) but disappear at
later stages of development (open arrow in H). (I,J) Changes in
the OD are associated with a strong decrease in the number of
Math5-positive RGC precursors in the orJ retinas
(J) when compared with wild-type embryos (I). Scale bar: 50 µm in A-F; 100
µm in G,H; 200 µm in I,J.
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